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Descripción
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| Our group is interested in the study of the role of the transporters involved in maintaining the Na+ and K+ homeostasis in fungi and plant cells. For a long time, we have used the yeast Saccharomyces cerevisiae as a model organism to clone and functionally characterize Na+ and K+ transporters expressing in different cellular compartments, initially, its own transporters and later, homologous transporters of others fungi and plants, from the primitive moss Physcomitrella patens to several monocot plants. One aspect that lacks so far is to know whether any of these Na+ and K+ transporters have any relevant role during symbiotic associations. The current goal of our project is to identify the Na+ and K+ transport systems that operate during the symbiosis between plants and microorganisms associated and to determine their contribution to the mineral nutrition and salt tolerance of plants growing in saline conditions. The model organisms used are the endophyte Piriformospora indica in interaction with rice. In a first attempt we have initiated the characterization of the ENA ATPases of the fungus. These are proteins involved in Na+ efflux from cells that have demonstrated to be very effective systems to increase salt tolerance in fungi. Two genes, PiENA1 and PiENA5 have been identified in the Piriformospora genome (https://genome.jgi.doe.gov/Pirin1/Pirin1.home.html). Their cloning and heterologous expression in yeast defective mutants in their own Na+ efflux systems indicated that PiENA1 preferentially transports K+ and PiENA5 Na+. Both genes are constitutively expressed but at very low levels in Piriformospora growing in control conditions and both are induced in saline conditions and during the fungal growth at alkaline pH. The inoculation of Arabidopsis and rice roots with the fungus induced an increase in the growth of both the roots and aerial part of the plants growing in control medium but also exposed to salinity. During infection, PiENA1 and PiENA5 are expressed. Now we want to studied a putative role of these transporters in the increased salt tolerance of the infected plants. Funded by grant AGL2016-80593-R and BIO2014-56153-REDT from MINECO. | |
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Internacional
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No |
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Nombre congreso
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XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |
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Tipo de participación
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970 |
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Lugar del congreso
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Salamanca |
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Revisores
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Si |
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ISBN o ISSN
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000-0-00-000000-0 |
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DOI
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Fecha inicio congreso
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04/07/2018 |
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Fecha fin congreso
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06/07/2018 |
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Desde la página
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212 |
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Hasta la página
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212 |
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Título de las actas
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Libro de abstracts de la XIV REUNIÓN DE BIOLOGÍA MOLECULAR DE PLANTAS |