|
Descripción
|
|
|---|---|
| IDENTIFICATION OF TRANSPORT SYSTEM INVOLVED IN ROOT Na+ UPTAKE THROUGH TRANSCRIPTOME PROFILING Rocío Álvarez-Aragón, Lorena Blázquez and Begoña Benito Centro de Biotecnología y Genómica de Plantas (CBGP), Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Campus Montegancedo UPM. 28223-Pozuelo de Alarcón (Madrid), Spain. Despite intensive research for more than 50 years, how Na+ enters the plant is still unknown. Previously we found out that under saline conditions Na+ entrance in Arabidopsis takes place largely in a nitrate-dependent manner (Álvarez-Aragón and Rodriguez-Navarro, 2017), suggesting that Na+ may enter the root through a nitrate transporter. A large family of at least 53 genes in Arabidopsis encodes nitrate transporters. Therefore, the identification of which transporters are involved in Na+ uptake may be a hard task. In this study we performed a transcriptomic analysis to identify transporters differentially expressed during Na+ root entry. Using Illumina RNA-Seq transcriptome profiling, we compared the expression of genes encoding putative transporters or channels in hydroponic-grown plants in control conditions (MES without Na+) or in two different saline conditions, nitrate independent Na-MES (MES with 20 mM Na+) or nitrate dependent NaNO3 (20 mM NO3 and 20 mM Na+). A total of 49 and 57 differentially expressed genes (DEGs) were identified under Na-MES and NaNO3 treatments compared to control with less than 20% overlap between both gene sets. Indeed, 42 DEGs encoding transporters were identified in a comparison between NaNO3 and Na-MES conditions. We hypothesized that the transporters responsible for nitrate-dependent Na+ entrance will be among this latter group and therefore we have selected some genes for further study. In addition, we also identified candidates for nitrate-independent Na+ uptake among DEGs from Na-MES vs control comparison. We are carrying out a phenotypic study of Na+ accumulation of the respective Arabidopsis defective mutants and a functional characterization in Na+ defective yeast mutants that should allow us to identify the transporters involved in the Na+ root entrance by plant roots. References Álvarez-Aragón and Rodríguez-Navarro, 2017. The Plant Journal, 91: 208-219 Funded by grants AGL2012-36174, AGL2016-80593-R and BIO2014-56153-REDT from MINECO. | |
|
Internacional
|
Si |
|
Comentarios Mérito
|
|