Memorias de investigación
Artículos en revistas:
HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism
Año:2012
Áreas de investigación
  • Biología celular
Datos
Descripción
We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5¢ region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3¢ region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Journal
ISSN
0960-7412
Factor de impacto JCR
6,948
Información de impacto
Datos JCR del año 2010
Volumen
71
DOI
Número de revista
Desde la página
750
Hasta la página
762
Mes
SIN MES
Ranking
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Participantes
  • Autor: Ronald J.F.J. Oomen Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, France
  • Autor: Begoña Benito Casado UPM
  • Autor: Hervé Sentenac Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, Franc
  • Autor: Alonso Rodriguez Navarro UPM
  • Autor: Manuel Talon Instituto Valenciano de Investigaciones Agrarias, Valencia
  • Autor: Anne-Allienor Very Biochimie et physiologie Moleculaire des Plantes, Institut de Biologie Integrative des Plantes, INRA/Montpellier SupAgro/Universite Montpellier, Franc
  • Autor: Concha Domingo Instituto Valenciano de Investigaciones Agrarias, Valencia
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Homeostasis iónica y ciclo celular
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología